Talwalkar played various side roles in popular Marathi television shows like Awantika and Asambhav. In 2007, she acted in the film Chandrakant Kulkarni. Her 2008 film Tuzya Mazyat was loosely based on the 1998 English film Stepmom. Talwalkar played second wife of Shashank (played by Sachin Khedekar) who is earlier married to Asmita (played by Mrinal Kulkarni) and also has two children. In 2009, she played the lead role in the film Tinhisaanja. The film was about the generation gap and taking care of one's older parents. Sandeep Kulkarni played her husband and her in-laws were played by actors Ramesh Deo and Ashalata Wabgaonkar. Her 2012 film Shyamche Vadil saw her playing the lead role of Leena, a shrewd wife who harasses her husband for not bringing the money for the household. After seeing such harassment for 22 years, their son Shyam decides to fight for his father's rights claiming their divorce. The role of father Madhav is played by Tushar Dalvi and of Shyam by debutant Chinmay Udgirkar.